Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA.
Nat Nanotechnol. 2010 Dec;5(12):868-73. doi: 10.1038/nnano.2010.213. Epub 2010 Nov 14.
It has been proposed that single molecules of DNA could be sequenced by measuring the physical properties of the bases as they pass through a nanopore. Theoretical calculations suggest that electron tunnelling can identify bases in single-stranded DNA without enzymatic processing, and it was recently experimentally shown that tunnelling can sense individual nucleotides and nucleosides. Here, we report that tunnelling electrodes functionalized with recognition reagents can identify a single base flanked by other bases in short DNA oligomers. The residence time of a single base in a recognition junction is on the order of a second, but pulling the DNA through the junction with a force of tens of piconewtons would yield reading speeds of tens of bases per second.
有人提出，通过测量碱基穿过纳米孔时的物理特性，有可能对单分子 DNA 进行测序。理论计算表明，电子隧穿无需酶处理即可识别单链 DNA 中的碱基，最近的实验表明，隧穿可以感知单个核苷酸和核苷。在这里，我们报告说，用识别试剂功能化的隧穿电极可以识别短 DNA 寡聚物中其他碱基侧翼的单个碱基。单个碱基在识别结中的停留时间约为一秒钟，但如果用数十皮牛顿的力将 DNA 拉过结，那么读取速度将达到每秒数十个碱基。
