X 射线显微镜解析的三维细胞超微结构。
Three-dimensional cellular ultrastructure resolved by X-ray microscopy.
机构信息
Helmholtz Zentrum Berlin für Materialien und Energie GmbH, Wilhelm-Conrad-Röntgen Campus, Berlin, Germany.
出版信息
Nat Methods. 2010 Dec;7(12):985-7. doi: 10.1038/nmeth.1533. Epub 2010 Nov 14.
Abstract
We developed an X-ray microscope using partially coherent object illumination instead of previously used quasi-incoherent illumination. The design permitted the incorporation of a cryogenic tilt stage, enabling tomography of frozen-hydrated, intact adherent cells. We obtained three-dimensional reconstructions of mouse adenocarcinoma cells at ∼36-nm (Rayleigh) and ∼70-nm (Fourier ring correlation) resolution, which allowed us to visualize the double nuclear membrane, nuclear pores, nuclear membrane channels, mitochondrial cristae and lysosomal inclusions.
摘要
我们开发了一种使用部分相干物光照明的 X 射线显微镜，而不是以前使用的准非相干照明。该设计允许低温倾斜台的结合，实现了冷冻水合完整贴壁细胞的断层摄影术。我们获得了约 36nm（瑞利）和约 70nm（傅里叶环相关）分辨率的小鼠腺癌细胞的三维重建，这使我们能够可视化双核膜、核孔、核膜通道、线粒体嵴和溶酶体内含物。
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