Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.
Mod Pathol. 2011 Feb;24(2):277-87. doi: 10.1038/modpathol.2010.202. Epub 2010 Nov 12.
Minichromosome maintenance protein 7 (MCM7) is involved in replicative licensing and synthesis of DNA. It was previously identified as an overexpressed gene in high-grade serous carcinomas compared with serous borderline tumors of the ovary in cDNA microarray studies. In this study, we sought to validate MCM7 expression in 342 ovarian tumors on tissue microarrays. MCM7 expression was quantified as the MCM7 labeling index, and it was independently generated by two methods: a score provided by manual review of each sample by a pathologist observer and by an automated cellular imaging system. Analyses of MCM7 scores indicated a high degree of concordance and distribution between the observer- and machine-generated MCM7 labeling indexes. MCM7 expression was significantly higher in high-grade serous carcinomas than in serous borderline tumors or other histological subtypes of ovarian cancer. For both observer- and machine-derived scores, univariate analyses indicated the significant association of a high MCM7 labeling index with better progression-free survival in high-grade serous carcinomas. These results suggest the clinical importance of MCM7 expression in high-grade serous carcinomas of the ovary and the need for further evaluation of MCM7 as a potential prognostic factor in ovarian cancer.
微染色体维持蛋白 7（MCM7）参与复制许可和 DNA 的合成。在 cDNA 微阵列研究中，与卵巢浆液性交界性肿瘤相比，它被先前鉴定为高级别浆液性癌中过表达的基因。在这项研究中，我们试图在组织微阵列上验证 342 例卵巢肿瘤中的 MCM7 表达。MCM7 表达被量化为 MCM7 标记指数，并通过两种方法独立生成：病理学家观察者手动评估每个样本的评分和自动化细胞成像系统。MCM7 评分分析表明观察者和机器生成的 MCM7 标记指数之间具有高度的一致性和分布。MCM7 在高级别浆液性癌中的表达明显高于浆液性交界性肿瘤或其他卵巢癌组织学亚型。对于观察者和机器衍生的评分，单因素分析表明，高 MCM7 标记指数与高级别浆液性癌的无进展生存时间显著相关。这些结果表明 MCM7 表达在卵巢高级别浆液性癌中的临床重要性，并需要进一步评估 MCM7 作为卵巢癌潜在的预后因素。
