Kaergel E, Aoyama Y, Schunck W H, Mueller H G, Yoshida Y
Central Institute for Molecular Biology, National Academy of Science of G.D.R., Berlin-Buch.
Yeast. 1990 Jan-Feb;6(1):61-7. doi: 10.1002/yea.320060107.
The occurrence of cytochrome P-450(14DM) (lanosterol 14 alpha-demethylase) and cytochrome P-450alk (long-chain alkane terminal hydroxylase) in various yeast strains was determined with immunological procedures. Cytochrome P-450(14DM), which is constitutive or housekeeping enzyme playing an essential role in ergosterol biogenesis, was found in all yeast strains so far tested. Cytochromes P-450(14DM) from different species of yeast were immunologically different, although they may have had a few common antigenic sites. In contrast, cytochrome P-450alk was detected only in the alkane-assimilating yeasts.
采用免疫学方法测定了各种酵母菌株中细胞色素P-450(14DM)(羊毛甾醇14α-脱甲基酶)和细胞色素P-450alk(长链烷烃末端羟化酶)的存在情况。细胞色素P-450(14DM)是一种组成型或管家酶，在麦角固醇生物合成中起重要作用，在迄今为止测试的所有酵母菌株中均有发现。不同酵母物种的细胞色素P-450(14DM)在免疫学上是不同的，尽管它们可能有一些共同的抗原位点。相比之下，仅在能同化烷烃的酵母中检测到细胞色素P-450alk。
