National Institute of Science Education and Research (NISER) IOP Campus, P.O.:Sainik School, Bhubaneswar. 751005, Orissa, India.
Org Biomol Chem. 2011 Feb 7;9(3):725-9. doi: 10.1039/c0ob00528b. Epub 2010 Nov 15.
Nucleic (DNA) acids having contiguous stretch of G sequence form quadruplex structure, which is very critical to control cell division. Recently the existence of G-quadruplex in RNA is also reported in presence of monovalent metal ion. PNA is a promising DNA analogue which binds strongly to DNA to form PNA:DNA duplex or PNA(2):DNA triplex. PNA also forms quadruplexes such G-quadruplex and i-motif in G and C-rich sequences respectively. aep-PNA containing a prolyl ring is one of several PNA analogues that provide rigidity and chirality in backbone and has binding affinity to natural DNA which is higher than that of PNA. Here we examine the ability of aep-PNA-G to form a quadruplex by UV, CD and mass spectroscopic techniques.
具有连续 G 序列的核酸（DNA）形成四链体结构，这对控制细胞分裂非常关键。最近，在存在单价金属离子的情况下，也有报道称 RNA 中存在 G-四链体。PNA 是一种很有前途的 DNA 类似物，它能与 DNA 强烈结合形成 PNA:DNA 双链体或 PNA(2):DNA 三链体。PNA 还能在富含 G 和 C 的序列中分别形成 G-四链体和 i- 发夹结构。含脯氨酸环的 aep-PNA 是几种提供刚性和手性的 PNA 类似物之一，其对天然 DNA 的结合亲和力高于 PNA。在这里，我们通过紫外、圆二色和质谱技术研究了 aep-PNA-G 形成四链体的能力。
