Amato Jussara, Gabelica Valérie, Borbone Nicola, Rosu Frédéric, De Pauw Edwin, Oliviero Giorgia, Piccialli Gennaro, Mayol Luciano
Dipartimento di Chimica delle Sostanze Naturali, Università degli Studi di Napoli Federico II, Via D. Montesano 49, I-80131 Napoli, Italy.
Nucleic Acids Symp Ser (Oxf). 2008(52):167-8. doi: 10.1093/nass/nrn085.
In this work we investigated the interaction between the short ac(4)a C-rich peptide nucleic acid (PNA) probe and two intramolecular G-quadruplex targets having the same G-tetrad core, but different folding topologies. The T(G(4)T)(3)G(4)T and the recently reported tetra-end-linked-(TG(4)T)(4) G-rich oligonucleotides (GROs) were chosen and synthesized for this study. UV, CD, and MS experiments revealed the formation of novel 1:1 G-quadruplex-PNA complexes besides the expected DNA-PNA heteroduplexes.
在本研究中，我们探究了短的富含ac(4)a C的肽核酸（PNA）探针与两个具有相同G-四联体核心但折叠拓扑结构不同的分子内G-四联体靶标之间的相互作用。为此研究选择并合成了T(G(4)T)(3)G(4)T和最近报道的四末端连接的-(TG(4)T)(4)富含G的寡核苷酸（GROs）。紫外、圆二色光谱和质谱实验表明，除了预期的DNA-PNA异源双链体之外，还形成了新型的1:1 G-四联体-PNA复合物。
