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用从异常毕赤酵母 NCYC 434 中分离得到的新型外切-β-1,3-葡聚糖酶 Panomycocin 抑制体外和体内的青霉属和意大利青霉。

Inhibition of penicillium digitatum and penicillium italicum in vitro and in planta with Panomycocin, a novel exo-β-1,3-glucanase isolated from Pichia anomala NCYC 434.

机构信息

Department of Biology, TED Ankara College Foundation High School, Incek, Gölbaşı, 06830, Ankara, Turkey.

出版信息

Antonie Van Leeuwenhoek. 2011 Jan;99(1):85-91. doi: 10.1007/s10482-010-9527-0. Epub 2010 Nov 14.

DOI:10.1007/s10482-010-9527-0
PMID:21076971
Abstract

Panomycocin, a novel exo-beta 1,3 glucanase, was tested as an antifungal agent against green and blue mold diseases, the most important causes of post harvest decay in citrus fruits. All tested isolates of Penicillium digitatum and Penicillium italicum were susceptible to panomycocin in vitro. Effective panomycocin concentrations for 50% growth inhibition (MIC-2) for P. digitatum and P. italicum were 2 and 1 microg ml(-1), respectively. Complete (MIC-0) growth inhibition of all isolates observed at a panomycocin concentration of 16 microg ml(-1). Treatment of spores with panomycocin at values lower than the MIC-0 led to slower germ tube elongation and mycelium growth. In tests on fruit, panomycocin at concentrations equal to in vitro MIC-0 value protected lemon fruit from decay.

摘要

泛霉素是一种新型的外切-β-1,3-葡聚糖酶,被测试作为一种抗真菌剂,用于防治柑橘果实采后绿霉病和青霉病,这两种病害是造成柑橘腐烂的最重要原因。所有测试的桔青霉素和意大利青霉分离物在体外都易受泛霉素的影响。桔青霉素和意大利青霉的有效泛霉素浓度为 50%生长抑制(MIC-2),分别为 2 和 1μg/ml。在 16μg/ml 的泛霉素浓度下,观察到所有分离物的完全(MIC-0)生长抑制。用低于 MIC-0 的泛霉素处理孢子,导致发芽管伸长和菌丝生长缓慢。在果实试验中,MIC-0 值的泛霉素浓度可保护柠檬果实不腐烂。

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