Department of Life Sciences, Salish Kootenai College, Pablo, Montana, USA.
Energy Keepers Inc., Polson, Montana, USA.
Microbiol Spectr. 2024 Oct 3;12(10):e0044424. doi: 10.1128/spectrum.00444-24. Epub 2024 Aug 20.
A yeast-like extremophile organism, , has been isolated from the superfund site the Berkeley Pit Lake in Butte, Montana. Studies demonstrate growth in some of the known Berkeley Pit Lake solutes. Microbial growth dynamics under controlled conditions were compared of for multiple metal concentrations. Each solute/metal was tested separately at previously reported concentrations on the geochemistry of the Berkeley Pit lake in the first 0.2 m in spring (pH 2.5). grew well with sulfur (S), MgSO, CaSO, potassium chloride (KCl), and NaSO and was inhibited with FeSO, MnSO, CuSO, AlSO, or ZnSO. With the addition of elemental S, growth was observed for FeSO indicating minimal growth rescue. PCR amplification of genomic DNA from the organism using known ribosomal primers indicates the strain to be ATCC8168 (CBS 5759). From this data, it can be concluded that ATCC8168 from the Berkeley Pit is an extremophile that exhibits metal-specific growth.IMPORTANCELaboratory growth studies of a strain of from the Berkeley Pit have found the organism to be metal specific indicating some unique metabolism possibilities. These studies show that this strain is metal-dependent and provides information about the adaptable tolerance of organisms in superfund sites as well as giving a basis for future bioremediation development utilizing .
已从蒙大拿州比尤特的超级基金场地伯克利坑湖分离出一种酵母样极端微生物。研究表明，在一些已知的伯克利坑湖溶质中能够生长。在受控条件下比较了 的微生物生长动力学，以研究多种金属浓度下的情况。每种溶质/金属都在以前报道过的浓度下进行了单独测试，这些浓度与伯克利坑湖的地球化学有关，测试时间是春季第一个 0.2 米（pH2.5）。 在硫（S）、MgSO、CaSO、氯化钾（KCl）和 NaSO 中生长良好，而在 FeSO、MnSO、CuSO、AlSO 或 ZnSO 中受到抑制。添加元素 S 后，FeSO 的生长得到观察，表明最小化的生长恢复。使用已知核糖体引物对该生物体的基因组 DNA 进行 PCR 扩增表明该菌株为 ATCC8168（CBS5759）。根据这些数据，可以得出结论，来自伯克利坑的 ATCC8168 是一种极端微生物，表现出对金属的特异性生长。
从伯克利坑分离出的一株 的实验室生长研究发现，该生物体对金属具有特异性，表明其具有一些独特的代谢可能性。这些研究表明，该菌株是金属依赖性的，并提供了有关超级基金场地中生物体适应性耐受的信息，以及为未来利用 进行生物修复开发提供了基础。
