National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
Electrophoresis. 2010 Nov;31(22):3658-64. doi: 10.1002/elps.201000335.
In Part 1 of this work, a theoretical description of gradient elution moving boundary electrophoresis (GEMBE) is presented along with a comparison between GEMBE and the more conventional technique of CZE. In this second part, experimental results are presented for comparison with the theoretical predictions of Part 1. Results for the step width, spacing, and resolution are examined as a function of the salient parameters affecting the results of a GEMBE separation (starting pressure, counterflow acceleration, and electric field strength).
在这项工作的第一部分中，提出了梯度洗脱移动边界电泳（GEMBE）的理论描述，并将 GEMBE 与更传统的 CZE 技术进行了比较。在第二部分中，给出了实验结果，以便与第一部分的理论预测进行比较。考察了作为影响 GEMBE 分离结果的显著参数（起始压力、逆流加速和电场强度）的函数的步宽、间隔和分辨率的结果。
