Gavrilova S A, Morozova M P, Kniazhentseva A K, Pogodina L S, Postnikov A B, Chentsov Iu S
Izv Akad Nauk Ser Biol. 2010 Sep-Oct(5):597-609.
The development of autoimmune myocarditis in rats after a single hypodermic injection of rat myosin mixed with a complete Freund's adjuvant (CFA) (400 microg/kg in 200 microl) was studied. The rats from the control group were injected with only CFA. The titer of antibodies to myosin, infiltration of lymphocytes into the myocardium, ultrastructural damage of myofibrils, mitochondria, and nuclei of cardiomyocytes were maximally pronounced on days 14-21 after the immunization with myosin, which indicates a peak of the inflammatory reaction. The content of nitrites and nitrates in the blood serum and myocardium of immunized rats were also studied. A certain contribution to the development of the inflammation is made by CFA: in rats injected with only CFA, morphological signs of myocarditis were found, but to a much lesser degree than in the group immunized with myosin.
研究了单次皮下注射与完全弗氏佐剂（CFA）混合的大鼠肌球蛋白（400微克/千克，溶于200微升）后大鼠自身免疫性心肌炎的发展情况。对照组大鼠仅注射CFA。免疫后第14至21天，针对肌球蛋白的抗体滴度、淋巴细胞浸润到心肌、肌原纤维、线粒体和心肌细胞核的超微结构损伤最为明显，这表明炎症反应达到高峰。还研究了免疫大鼠血清和心肌中亚硝酸盐和硝酸盐的含量。CFA对炎症的发展有一定作用：仅注射CFA的大鼠出现了心肌炎的形态学迹象，但程度远低于肌球蛋白免疫组。
