Morozova M P, Gavrilova S A, Zemtsova L V, Pogodina L S, Postnikov A B, Chentsov Iu S
Ross Fiziol Zh Im I M Sechenova. 2012 Feb;98(2):269-82.
Myocarditis development was investigated after immunization rats with single subcutaneous injection of cardiac myosin (800 microg/kg) with incomplete Freund's adjuvant (IFA) (M + IFA group). Control group received equal volume of IFA alone or nothing (intact group). On days 4, 14, and 21 after injection, light and electron microscopy of heart sections, morphometric analysis, estimation of proinflammatory cytokines (IL-1p, IL-6, VEGF, TNFa and iNOS) expression were used to evaluate inflammatory response in myocardium. In addition, we estimated cardiac myosin antibody levels in blood serum and nitrite and nitrate levels in blood serum. Our data showed that immunization with cardiac myosin combined with IFA led to inflammatory response in the rat myocardium. Acute inflammation (i.e. lymphocyte infiltration of myocardium and increase of proinflammatory cytokines level) in M + IFA group occurred on 21 days after immunization.
通过给大鼠单次皮下注射心肌肌凝蛋白（800微克/千克）与不完全弗氏佐剂（IFA）（M + IFA组）来研究心肌炎的发展。对照组单独接受等量的IFA或不接受任何处理（完整组）。在注射后的第4天、14天和21天，使用心脏切片的光镜和电镜检查、形态计量分析、促炎细胞因子（IL-1β、IL-6、VEGF、TNFα和iNOS）表达的评估来评价心肌中的炎症反应。此外，我们还测定了血清中心肌肌凝蛋白抗体水平以及血清中亚硝酸盐和硝酸盐水平。我们的数据表明，心肌肌凝蛋白与IFA联合免疫导致大鼠心肌出现炎症反应。M + IFA组在免疫后21天出现急性炎症（即心肌淋巴细胞浸润和促炎细胞因子水平升高）。
