Jaeger L A, Lamar C H, Cline T R, Cardona C J
Department of Anatomy, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907.
Am J Vet Res. 1990 Mar;51(3):471-4.
The effect of ingested epidermal growth factor (EGF) on the small intestinal mucosa of conventionally weaned pigs was determined. At 21 days of age, 39 pigs were randomly distributed into suckling and weaned treatment groups that were administered 124 micrograms of EGF, 372 micrograms of EGF, or the dosing compound daily. Fecal water content was determined daily. On postweaning days 0, 3, 6, and 9, representative pigs from each group were euthanatized, and jejunal mucosa samples were collected for determination of villus-to-crypt ratio, total protein content, disaccharidase activities, and microbiological populations. At postweaning day 3, the 372-micrograms dose of EGF significantly (P less than or equal to 0.05) increased jejunal lactase and sucrase activities in the weaned pigs. Increased lactase activity was not greater than that of the suckling pig controls, whereas sucrase activity was significantly (P less than or equal to 0.05) higher than that of the suckling pig controls. Significant changes were not observed in villus-to-crypt ratio, mucosal protein content, or disaccharidase activities on other collection days.
研究了摄入表皮生长因子（EGF）对传统断奶仔猪小肠黏膜的影响。在21日龄时，将39头猪随机分为哺乳和断奶治疗组，分别每日给予124微克EGF、372微克EGF或给药复合物。每天测定粪便含水量。在断奶后第0、3、6和9天，对每组的代表性猪实施安乐死，并采集空肠黏膜样本，以测定绒毛与隐窝比例、总蛋白含量、双糖酶活性和微生物种群。在断奶后第3天，372微克剂量的EGF显著（P≤0.05）提高了断奶仔猪空肠乳糖酶和蔗糖酶的活性。乳糖酶活性的增加不超过哺乳仔猪对照组，而蔗糖酶活性显著（P≤0.05）高于哺乳仔猪对照组。在其他采集日，未观察到绒毛与隐窝比例、黏膜蛋白含量或双糖酶活性有显著变化。
