Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.
Biochemistry (Mosc). 2010 Sep;75(9):1132-8. doi: 10.1134/s0006297910090063.
Lipid contents were studied in tissue and nuclei isolated from neurons and glia of neocortex of rats under conditions of normothermia and in the state of artificial hypobiosis caused by hypothermia-hypoxia-hypercapnia. Compared to the neocortex tissue, both nuclear fractions were fivefold impoverished in phospholipids and cholesterol and strongly enriched with mono- and diglycerides and fatty acids. The nuclear fractions from neurons and glia contained similar amounts of phospholipids, and only the cardiolipin content in the neuronal nuclei was lower than in the glial nuclei. The state of artificial hypobiosis in rats led to an increase in the cholesterol/phospholipids ratio (mol/mol) in the nuclei from the neurons and glia; amounts of cholesterol and sphingomyelin in the nuclei from the glia were increased. The increases in the cholesterol and sphingomyelin contents and in the cholesterol/phospholipids ratio suggest an involvement of lipid-dependent signaling systems of the nuclei in the functional response of mammalian neocortex cells to artificial hypobiosis.
在正常体温和低温-缺氧-高二氧化碳引起的人工低代谢状态下，研究了大鼠新皮质神经元和神经胶质细胞分离的组织和核中的脂质含量。与新皮质组织相比，核分数在磷脂和胆固醇方面减少了五倍，而单甘油酯和二甘油酯以及脂肪酸则强烈富集。神经元和神经胶质细胞的核分数含有相似量的磷脂，只有神经元核中的心磷脂含量低于神经胶质核。在大鼠中，人工低代谢状态导致神经元和神经胶质细胞核中的胆固醇/磷脂比值（摩尔/摩尔）增加；神经胶质细胞核中的胆固醇和神经鞘磷脂含量增加。胆固醇和神经鞘磷脂含量以及胆固醇/磷脂比值的增加表明，核中的脂质依赖信号系统参与了哺乳动物新皮质细胞对人工低代谢的功能反应。
