College of Animal Science and Technology, China Agricultural University, Beijing, China.
J Anim Breed Genet. 2010 Dec;127(6):474-80. doi: 10.1111/j.1439-0388.2010.00877.x. Epub 2010 Oct 28.
To investigate the genetic variations of mtATP6 and mtATP8 genes among different Chinese pig breeds, two fragments of 425 and 743 bp containing the whole coding region of mtATP8 and mtATP6 genes were amplified with 805 individuals from 23 Chinese local pig breeds, three types of Chinese wild boars and three European pig breeds. Sequence comparison identified a total number of 17 substitutions including six variable sites in mtATP8 and eleven substitutions in mtATP6 gene. The restriction enzyme Fok I revealed four polymorphic sites (nt8086, 8176, 8514 and 7784), and four RFLP haplotype patterns (A, B, C and D) were identified in mtATP6 and mtATP8 genes among all tested samples. Our data showed AC combined haplotype originated from Asia and BD was regarded as European origin. The average frequency of Asian mtDNA haplotypes was 38.3% across the investigated European breeds but varied within each breeds (13.3∼76.7%). Phylogenetic analyses were performed also considering some published sequences in the databases; the sequences were divided into three distinct groups, denoted A, E1, and E2. The Asian AC haplotype existed among the European domestic pigs was fully consistent with the results of previous molecular studies and well-documented history. This study will help us to better understand the genetic variations of mitochondrial genes among different Chinese pig breeds.
为了研究 mtATP6 和 mtATP8 基因在不同中国猪种中的遗传变异，我们用 805 个来自 23 个中国本地猪种、三种中国野猪和三种欧洲猪种的个体扩增了包含 mtATP8 和 mtATP6 基因全长编码区的两个 425 和 743 bp 的片段。序列比较确定了总共 17 个取代，包括 mtATP8 中的 6 个可变位点和 mtATP6 基因中的 11 个取代。限制性内切酶 Fok I 显示了 4 个多态性位点（nt8086、8176、8514 和 7784），在 mtATP6 和 mtATP8 基因中，在所有检测到的样本中发现了 4 种 RFLP 单倍型模式（A、B、C 和 D）。我们的数据显示，AC 组合单倍型起源于亚洲，BD 被认为是欧洲起源。在调查的欧洲品种中，亚洲 mtDNA 单倍型的平均频率为 38.3%，但在每个品种内有所不同（13.3∼76.7%）。还考虑了数据库中一些已发表的序列进行了系统发育分析；这些序列分为三个不同的组，分别表示为 A、E1 和 E2。存在于欧洲家猪中的亚洲 AC 单倍型与以前的分子研究结果和有据可查的历史完全一致。这项研究将有助于我们更好地了解不同中国猪种中线粒体基因的遗传变异。
