Brodie Laboratory for Craniofacial Genetics, University of Illinois at Chicago, 801 South Paulina Street, Chicago, IL 60612, USA.
Gene. 2011 Mar 1;473(2):92-9. doi: 10.1016/j.gene.2010.11.001. Epub 2010 Nov 13.
The maintenance and differentiation of embryonic stem cells (ES cells) depends on the regulation of gene expression through the coordinated binding of transcription factors to regulatory promoter elements. One of the genes involved in embryonic development is the chromatin factor CP27. Previously, we have shown that NF-Y interacted with the CP27 proximal promoter CCAAT-box. Here we report that CP27 gene expression in mouse ES cells is controlled by CCAAT and E-box cis-acting regulatory elements and their corresponding transcription factors NF-Y and USF1. Specifically, USF1 interacts with the E-box of the CP27 proximal promoter and NF-Y interacts with the CCAAT-box. NF-Y and USF1 also interacted with each other and activated the CP27 promoter in a synergistic fashion. Together, these studies demonstrate that gene expression of the chromatin factor CP27 is regulated through the interaction of the transcription factors NF-Y and USF1 with the CP27 proximal promoter.
胚胎干细胞（ES 细胞）的维持和分化依赖于通过转录因子与调控启动子元件的协调结合来调节基因表达。参与胚胎发育的基因之一是染色质因子 CP27。以前，我们已经表明 NF-Y 与 CP27 近端启动子 CCAAT 盒相互作用。在这里，我们报告说，在小鼠 ES 细胞中，CP27 基因的表达受 CCAAT 和 E 盒顺式作用调节元件及其相应的转录因子 NF-Y 和 USF1 控制。具体而言，USF1 与 CP27 近端启动子的 E 盒相互作用，而 NF-Y 与 CCAAT 盒相互作用。NF-Y 和 USF1 也相互作用，并以协同方式激活 CP27 启动子。总之，这些研究表明，染色质因子 CP27 的基因表达是通过转录因子 NF-Y 和 USF1 与 CP27 近端启动子的相互作用来调节的。
