Dipartimento di Bioscienze, Università degli Studi di Milano, Milano, Italy.
Dipartimento di Scienze della Vita e Biologia dei Sistemi, Università degli Studi di Torino, Torino, Italy.
Genomics. 2024 Sep;116(5):110895. doi: 10.1016/j.ygeno.2024.110895. Epub 2024 Jul 23.
NF-Y is a Transcription Factor that regulates transcription through binding to the CCAAT-box. To understand its strategy, we analyzed 16 ChIP-seq datasets from human and mouse cells. Shared loci, mostly located in promoters of expressed genes of cell cycle, metabolism and gene expression pathways, are associated with histone marks of active chromatin and specific modules of TFs. Other peaks are in enhancers and Transposable Elements -TE- of retroviral origin in human and mouse. We evaluated the relationship with USF1, a common synergistic partner in promoters and MLT1 TEs, upon NF-YB inactivation: USF1 binding decreases in promoters, modestly in MLT1, suggesting a pioneering role of NF-Y in formers, not in the latters. These data define a common set of NF-Y functional targets across different mammalian cell types, suggesting a pioneering role in promoters with respect to TEs.
NF-Y 是一种转录因子，通过与 CCAAT 盒结合来调节转录。为了了解其策略，我们分析了来自人和小鼠细胞的 16 个 ChIP-seq 数据集。共享的基因座主要位于细胞周期、代谢和基因表达途径中表达基因的启动子中，与活性染色质的组蛋白标记和 TF 的特定模块相关。其他峰位于人和小鼠逆转录病毒起源的增强子和转座元件（TE）中。我们评估了 NF-YB 失活时与常见协同伙伴 USF1 之间的关系：在启动子中，USF1 结合减少，在 MLT1 中适度减少，表明 NF-Y 在前者中具有先驱作用，而不是在后者中。这些数据定义了不同哺乳动物细胞类型中 NF-Y 功能靶标的常见集合，表明其在启动子中相对于 TE 具有先驱作用。
