Department of Dermatology, Yale University School of Medicine, P.O. Box 208059, New Haven, CT 06520-8059, USA.
J Immunol. 2010 Dec 15;185(12):7146-50. doi: 10.4049/jimmunol.1002163. Epub 2010 Nov 15.
The paucity of murine memory B cell markers has been a significant impediment to the study of memory. The most commonly used marker is IgG, which is neither sensitive nor specific, because activated nonmemory cells can be IgG(+), and memory cells can be IgM(+). In this article, we show that, together, PD-L2 (CD273), CD80, and CD73 define at least five phenotypic subsets of murine memory B cells. These subsets are generated from naive cells bearing a single BCR in response to a single T-dependent Ag. This diversity is independent of class switch, because IgG(1)- and IgM-bearing memory cells are found within each compartment. Memory subsets defined by PD-L2, CD80, and CD73 are biologically distinct from one another, because they differ in ontogeny and selection. Together, these distinctions suggest that there is a spectrum of memory B cells and progressive acquisition from more naive-like to more memory-like properties.
缺乏鼠类记忆 B 细胞标志物一直是研究记忆的一个重大障碍。最常用的标志物是 IgG，它既不敏感也不特异，因为激活的非记忆细胞可以是 IgG(+)，而记忆细胞可以是 IgM(+)。在本文中，我们表明，PD-L2（CD273）、CD80 和 CD73 一起至少可以定义五种表型不同的鼠类记忆 B 细胞亚群。这些亚群是由单一 BCR 上的幼稚细胞产生的，对单一 T 依赖性抗原有反应。这种多样性与类别转换无关，因为在每个隔室中都可以发现 IgG(1)-和 IgM-携带的记忆细胞。由 PD-L2、CD80 和 CD73 定义的记忆亚群在生物学上彼此不同，因为它们在个体发生和选择上存在差异。总之，这些差异表明存在记忆 B 细胞谱，并且从更类似于幼稚的特性逐渐获得更类似于记忆的特性。
