Mizuta Einosuke, Shirai Manabu, Arakawa Keita, Hidaka Kyoko, Miake Junichiro, Ninomiya Haruaki, Kato Masahiko, Shigemasa Chiaki, Shirayoshi Yasuaki, Hisatome Ichiro, Morisaki Takayuki
Division of Molecular Medicine and Therapeutics, Department of Multidisciplinary Internal Medicine, Faculty of Medicine, Tottori University, Yonago, Japan.
Biomed Res. 2010 Oct;31(5):301-5. doi: 10.2220/biomedres.31.301.
We investigated the distribution of T-type Ca(2+) channel mRNAs in the mouse embryonic heart. Cav3.2, but not Cav3.1, was expressed in the E8.5 embryonic heart along with cardiac progenitor markers (Nkx2.5, Tbx5, Isl-1) and contractile proteins (alpha and beta MHC). In the E10.5 heart, the distribution of Cav3.1 mRNA was confirmed in the AV-canal and overlapped with that of MinK or Tbx2. Cav3.2 mRNA was observed not only in the AV-canal but also in the outflow tract, along with MinK and Isl-1, indicating the expression of Cav3.2 in the secondary heart field. Thus, Cav3.2 may contribute to the development of the outflow tract from the secondary heart field in the embryonic heart, whereas Cav3.1 may be involved in the development of the cardiac conduction-system together with Cav3.2.
我们研究了T型Ca(2+)通道mRNA在小鼠胚胎心脏中的分布。在E8.5胚胎心脏中，Cav3.2而非Cav3.1与心脏祖细胞标志物（Nkx2.5、Tbx5、Isl-1）和收缩蛋白（α和β肌球蛋白重链）一同表达。在E10.5心脏中，Cav3.1 mRNA的分布在房室管中得到证实，且与MinK或Tbx2的分布重叠。Cav3.2 mRNA不仅在房室管中被观察到，还在流出道中与MinK和Isl-1一起被观察到，这表明Cav3.2在第二心脏场中表达。因此，Cav3.2可能有助于胚胎心脏中来自第二心脏场的流出道发育，而Cav3.1可能与Cav3.2一起参与心脏传导系统的发育。
