在口腔鳞状细胞癌细胞质中 MAC30 的过表达预示着淋巴结转移和低分化。

Overexpression of MAC30 in the cytoplasm of oral squamous cell carcinoma predicts nodal metastasis and poor differentiation.

机构信息

Department of Stomatology, Pathology, First Hospital, Hebei Medical University, Shijiazhuang, China.

出版信息

Chemotherapy. 2010;56(6):424-8. doi: 10.1159/000317582. Epub 2010 Nov 16.

DOI:10.1159/000317582

PMID:21079401

Abstract

BACKGROUND

Expression of the meningioma-associated protein (MAC30) was increased in several types of tumors, including esophageal, gastric and colon tumors, compared to normal tissue. MAC30 expression levels gradually increased from normal colorectal mucosa to primary colorectal cancer and colorectal cancer spreading to the lymph nodes. MAC30 expression was related to survival in patients with colorectal cancer. However, there is no study on MAC30 in oral squamous cell carcinoma (OSCC).

METHODS

Therefore, MAC30 expression in OSCC was investigated and possible associations of MAC30 expression with clinicopathological variables in OSCC have been analyzed. MAC30 expression was immunohistochemically examined in 20 normal oral mucosa and 43 OSCC specimens.

RESULTS

Expression levels of MAC30 in the cytoplasm markedly increased from normal oral epithelial cells to primary OSCC. Strong cytoplasmic staining was significantly higher in primary OSCC compared to normal oral mucosa samples (51 vs. 20%, p = 0.019). Furthermore, MAC30 expression levels in primary tumors of patients with lymph node metastasis exceeded levels in those without metastasis (65 vs. 35%, p = 0.048), and MAC30 expression in poorly differentiated tumors was higher than in well-differentiated ones (90 vs. 39%, p = 0.005).

CONCLUSION

Overexpression of MAC30 in the cytoplasm of OSCC may predict nodal metastasis and poor differentiation.

摘要

背景

脑膜瘤相关蛋白（MAC30）的表达在多种肿瘤中增加，包括食管、胃和结肠肿瘤，与正常组织相比。MAC30 表达水平从正常结直肠黏膜逐渐增加到原发性结直肠癌和结直肠癌转移到淋巴结。MAC30 表达与结直肠癌患者的生存有关。然而，在口腔鳞状细胞癌（OSCC）中尚未研究 MAC30。

方法

因此，研究了 MAC30 在 OSCC 中的表达，并分析了 MAC30 表达与 OSCC 临床病理变量的可能相关性。用免疫组织化学方法检测 20 例正常口腔黏膜和 43 例 OSCC 标本中的 MAC30 表达。

结果

MAC30 在细胞质中的表达水平从正常口腔上皮细胞到原发性 OSCC 明显增加。原发性 OSCC 中细胞质的强染色明显高于正常口腔黏膜样本（51%比 20%，p=0.019）。此外，有淋巴结转移的患者的原发性肿瘤中 MAC30 表达水平高于无转移的患者（65%比 35%，p=0.048），并且 MAC30 在低分化肿瘤中的表达高于高分化肿瘤（90%比 39%，p=0.005）。