Rabbani Hodjattallah, Ostadkarampour Mahyar, Danesh Manesh Amir Hossein, Basiri Abbas, Jeddi-Tehrani Mahmood, Forouzesh Flora
Dept. of Antigen and Antibody Engineering, Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Immune and Gene Therapy Lab., CCK, Dept. of Oncology-Pathology, Karolinska University Hospital Solna, Karolinska Institute, Stockholm, Sweden.
Iran Biomed J. 2010 Jul;14(3):77-82.
The ectopic expression of receptor tyrosine kinase Ror1 has been reported in patients with hematological malignancies such as chronic lymphocytic leukemia and acute lymphoblastic leukemia. Here we report, for the first time, expression of ROR1 gene in both tumor tissues and peripheral blood mononuclear cells (PBMC) from patients with renal cancer (RC).
In the current study, the expression of ROR1 gene was semi-quantitatively measured in PBMC and tumor tissues from 16 RC patients as well as PBMC from 22 healthy individuals relative to the expression of the housekeeping gene phosphoglucomutase 1 by RT-PCR.
Our results showed that ROR1 was expressed at gene level in 81.3 percent of renal tumor tissues (13 out of 16) whereas it was expressed in 94 percent of PBMC from RC patients (15 out of 16). A weak expression of ROR1 was observed in PBMC of 4 out of 22 healthy individual. A significant expression of ROR1 was observed in PBMC from RC patients when compared to that in PBMC from normal healthy individuals (P less than 0.001). The expression of ROR1 in PBMC may reflect a shedding of tumor cells into blood stream.
We conclude that detection of a high level of ROR1 expression in blood cells might assist in early detection of renal malignancies, providing taking into consideration the clinical symptoms of the disease.
已有报道称，受体酪氨酸激酶Ror1在慢性淋巴细胞白血病和急性淋巴细胞白血病等血液系统恶性肿瘤患者中存在异位表达。在此，我们首次报道了ROR1基因在肾癌（RC）患者的肿瘤组织和外周血单个核细胞（PBMC）中的表达情况。
在本研究中，通过逆转录聚合酶链反应（RT-PCR），相对于管家基因磷酸葡萄糖变位酶1的表达，对16例RC患者的PBMC和肿瘤组织以及22例健康个体的PBMC中ROR1基因的表达进行了半定量测定。
我们的结果显示，81.3%的肾肿瘤组织（16例中的13例）中ROR1在基因水平表达，而RC患者94%的PBMC（16例中的15例）中ROR1表达。22例健康个体中有4例的PBMC中观察到ROR1的弱表达。与正常健康个体的PBMC相比，RC患者的PBMC中观察到ROR1的显著表达（P小于0.001）。PBMC中ROR1的表达可能反映肿瘤细胞向血流中的脱落。
我们得出结论，考虑到该疾病的临床症状，检测血细胞中高水平的ROR1表达可能有助于肾癌的早期检测。
