Tolmach L J, Labanowska J
Department of Radiology, Washington University School of Medicine, St. Louis, Missouri 63108.
Cell Tissue Kinet. 1990 Mar;23(2):125-35. doi: 10.1111/j.1365-2184.1990.tb01339.x.
Shortening of the generation cycle in cells in which DNA synthesis had been temporarily inhibited in the previous generation, which has been reported several times in recent years, has been confirmed in HeLa cells. As in the previous studies, the shortening is attributable to accelerated transit of G1 resulting from the accumulation, during the inhibition, of a factor needed for initiation of DNA replication. It is shown that partial (85-96%) inhibition with any one of three inhibitors is effective when the inhibitor is added in G1 or in S, but more complete (99%) inhibition is effective only if the inhibitor is added after cells have entered S. In addition, cells begin to respond to the inhibition after a lag that increases as DNA synthesis in the early part of S is progressively inhibited with aphidicolin, indicating that competence to respond is achieved only after cells have reached a particular point in the replication of their genome.
近年来已有多次报道，上一代DNA合成被暂时抑制的细胞中，其代周期缩短现象在HeLa细胞中得到了证实。与之前的研究一样，这种缩短归因于在抑制期间积累的DNA复制起始所需因子导致的G1期加速过渡。结果表明，当三种抑制剂中的任何一种在G1期或S期添加时，部分（85 - 96%）抑制是有效的，但只有在细胞进入S期后添加抑制剂，更完全（99%）的抑制才有效。此外，细胞在经过一段滞后时间后开始对抑制产生反应，随着S期早期的DNA合成被阿非迪霉素逐渐抑制，滞后时间会增加，这表明只有在细胞达到其基因组复制的特定点后才获得反应能力。
