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DNA合成抑制剂在细胞周期的S期早期诱导姐妹染色单体交换。

Inhibitors of DNA synthesis induce sister chromatid exchanges at the early S phase of the cell cycle.

作者信息

Rainaldi G, Sessa M R, Mariani T

出版信息

Chromosoma. 1984;90(1):46-9. doi: 10.1007/BF00352277.

DOI:10.1007/BF00352277
PMID:6432492
Abstract

To investigate the origin of sister chromatid exchanges (SCEs) induced by inhibitors of DNA synthesis, V79/AP4 Chinese hamster cells were treated with aphidicolin, 1-beta-D-arabinofuranosylcytosine, and thymidine. At the end of the treatments we determined both the distribution of the cells in the various phases of the cell cycle and the induction of SCEs. Our data indicate that the cells that were replicating their DNA were arrested at various stages of the S phase. By analyzing the patterns of SCE distribution, we found that the metaphases of the treated cells exhibited either "normal" or enhanced levels of SCEs. Our results suggest that the inhibitors of DNA synthesis induce SCEs in the cells in early S phase probably by activation of potential replicative origins.

摘要

为了研究DNA合成抑制剂诱导的姐妹染色单体交换（SCEs）的起源，用阿非迪霉素、1-β-D-阿拉伯呋喃糖基胞嘧啶和胸腺嘧啶处理V79/AP4中国仓鼠细胞。在处理结束时，我们确定了细胞在细胞周期各阶段的分布以及SCEs的诱导情况。我们的数据表明，正在复制DNA的细胞在S期的不同阶段被阻滞。通过分析SCE分布模式，我们发现处理后细胞的中期呈现出“正常”或增强的SCE水平。我们的结果表明，DNA合成抑制剂可能通过激活潜在的复制起点在S期早期诱导细胞发生SCEs。

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Chromosoma. 1981;83(5):721-41. doi: 10.1007/BF00328530.
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Mutat Res. 1982 Mar;103(3-6):333-7. doi: 10.1016/0165-7992(82)90063-x.
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Synchronization of HeLa cell cultures by inhibition of DNA polymerase alpha with aphidicolin.通过用阿非迪霉素抑制DNA聚合酶α来同步化HeLa细胞培养物。
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Effects of inhibitors of DNA synthesis on spontaneous and ultraviolet light-induced sister-chromatid exchanges in Chinese hamster cells.DNA合成抑制剂对中国仓鼠细胞中自发的及紫外线诱导的姐妹染色单体交换的影响。
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