Hollingsworth N M, Goetsch L, Byers B
Department of Genetics, University of Washington, Seattle 98195.
Cell. 1990 Apr 6;61(1):73-84. doi: 10.1016/0092-8674(90)90216-2.
The HOP1 gene in Saccharomyces cerevisiae is important for meiotic chromosomal pairing, because hop1 diploids fail to form synaptonemal complex during meiosis and are defective in crossing over between, but not within, chromosomes. We demonstrate here that the HOP1 gene is transcriptionally regulated during sporulation and that the HOP1 protein is situated along the lengths of meiotic chromosomes. Furthermore, the HOP1 protein contains a Cys2/Cys2 zinc finger motif. A mutation within this motif that changes a cysteine to serine results in the hop1 phenotype, consistent with the possibility that the HOP1 gene product acts in chromosome synapsis by directly interacting with DNA. These observations demonstrate that HOP1 encodes a component of meiotic chromosomes, perhaps serving as a constituent of the synaptonemal complex.
酿酒酵母中的HOP1基因对减数分裂染色体配对很重要，因为hop1二倍体在减数分裂过程中无法形成联会复合体，并且在染色体之间而非染色体内的交叉互换存在缺陷。我们在此证明，HOP1基因在孢子形成过程中受到转录调控，并且HOP1蛋白沿减数分裂染色体的长度分布。此外，HOP1蛋白包含一个Cys2/Cys2锌指基序。该基序内将一个半胱氨酸突变为丝氨酸的突变会导致hop1表型，这与HOP1基因产物通过直接与DNA相互作用参与染色体联会的可能性一致。这些观察结果表明，HOP1编码减数分裂染色体的一个组分，可能作为联会复合体的一个组成部分。
