Department of Gynecology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, 88, Jiefang Road, Hangzhou 310009, Zhejiang, China.
Arch Gynecol Obstet. 2011 Apr;283(4):775-80. doi: 10.1007/s00404-010-1718-4. Epub 2010 Nov 16.
Our study is to investigate whether tyrosine kinase receptor B (TrkB) is expressed in eutopic endometrium of women with adenomyosis and its association with clinical characteristics.
We collected endometrial tissues from 31 women with adenomyosis and 30 adenomyosis-free women undergoing surgery for benign indications. TrkB expression was assessed by immunohistochemistry and reverse-transcription polymerase chain reaction.
Immunoreactive staining for TrkB was present as brown flocculent precipitate in the endometrial cells. The average level of TrkB protein (quantitation of immunostaining intensity) in secretory endometrial samples of women with adenomyosis was significantly higher than that in controls (p < 0.01). The average level of TrkB messenger RNA (mRNA) expression of women with adenomyosis was significantly higher than that of controls at secretory phase (p < 0.01). In addition, the immunostaining quantitation of TrkB protein was positively correlated with the serum CA125 (r = 0.308, p = 0.016) and dysmenorrhea (r = 0.393, p = 0.002).
Our study revealed elevation of TrkB protein and mRNA expression in the secretory endometrium of women with adenomyosis. Moreover, TrkB protein expression in human endometrium was positively correlated with the serum CA125 and dysmenorrhea. TrkB might contribute to the pathogenesis and progression of adenomyosis.
本研究旨在探讨酪氨酸激酶受体 B（TrkB）是否在子宫腺肌病患者的在位子宫内膜中表达及其与临床特征的关系。
我们收集了 31 名患有子宫腺肌病和 30 名无子宫腺肌病的因良性指征接受手术的妇女的子宫内膜组织。通过免疫组织化学和逆转录聚合酶链反应评估 TrkB 表达。
TrkB 的免疫反应性染色呈棕色絮状沉淀存在于子宫内膜细胞中。在分泌期子宫内膜样本中，患有子宫腺肌病的妇女的 TrkB 蛋白（免疫染色强度的定量）平均水平明显高于对照组（p<0.01）。患有子宫腺肌病的妇女在分泌期的 TrkB 信使 RNA（mRNA）表达水平明显高于对照组（p<0.01）。此外，TrkB 蛋白免疫染色定量与血清 CA125（r=0.308，p=0.016）和痛经（r=0.393，p=0.002）呈正相关。
我们的研究表明，在子宫腺肌病患者的分泌期子宫内膜中，TrkB 蛋白和 mRNA 表达升高。此外，人子宫内膜中 TrkB 蛋白的表达与血清 CA125 和痛经呈正相关。TrkB 可能有助于子宫腺肌病的发病机制和进展。
