Department of Nuclear Medicine, University of Munich (LMU), Munich, Germany.
Mol Imaging Biol. 2011 Dec;13(6):1204-14. doi: 10.1007/s11307-010-0439-1.
This study investigates the effects of (111)In-oxine incorporation on human mesenchymal stem cells' (hMSC) biology and viability, and the applicability of (111)In-oxine for single-photon emission computed tomography/X-ray computed tomography (SPECT/CT) monitoring of hMSC in vivo.
HMSC were labelled with 10 Bq/cell. Cellular retention of radioactivity, cell survival, and migration were evaluated over 48 h. Metabolic activity was assessed over 14 days and the hMSC's stem cell character was evaluated. Serial SPECT/CT was performed after intra-osseous injection to athymic rats over 48 h.
Labelling efficiency was 25%, with 61% of incorporated (111)In remaining in the hMSC at 48 h. The radiolabelling was without effect on cell viability, stem cell character, and plasticity, whereas metabolic activity and migration were significantly reduced. Grafted cells could be imaged in situ with SPECT/CT.
(111)In-oxine labelling moderately impaired hMSC's functional integrity while preserving their stem cell character. Combined SPECT/CT imaging of (111)In-oxine-labelled hMSC opens the possibility for non-invasive sequential monitoring of therapeutic stem cells.
本研究旨在探讨（111）In-oxine 掺入对人骨髓间充质干细胞（hMSC）生物学和活力的影响，以及（111）In-oxine 用于 hMSC 体内单光子发射计算机断层扫描/计算机断层扫描（SPECT/CT）监测的适用性。
以 10 Bq/细胞的浓度对 hMSC 进行标记。在 48 h 内评估放射性核素的细胞内保留率、细胞存活率和迁移率。在 14 天内评估代谢活性，并评估 hMSC 的干细胞特征。在 48 h 内对荷瘤无胸腺大鼠进行骨内注射后，进行连续 SPECT/CT 检查。
标记效率为 25%，48 h 时掺入的（111）In 中有 61%仍保留在 hMSC 中。放射性标记对细胞活力、干细胞特征和可塑性没有影响，而代谢活性和迁移能力显著降低。SPECT/CT 可原位成像移植细胞。
（111）In-oxine 标记适度损害了 hMSC 的功能完整性，同时保留了其干细胞特征。（111）In-oxine 标记的 hMSC 的 SPECT/CT 联合成像为治疗性干细胞的非侵入性连续监测开辟了可能性。
