Chin B B, Nakamoto Y, Bulte J W M, Pittenger M F, Wahl R, Kraitchman D L
Department of Radiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Nucl Med Commun. 2003 Nov;24(11):1149-54. doi: 10.1097/00006231-200311000-00005.
Mesenchymal stem cells (MSCs) have shown therapeutic potential if successfully delivered to the intended site of myocardial infarction. The purpose of this pilot study was to test the feasibility of 111In oxine labelling of MSCs and single photon emission computed tomography (SPECT) imaging after intravenous administration in a porcine model of myocardial infarction. Adult farm pigs (n=2) were subjected to closed chest experimental myocardial infarction. 111In oxine labelled MSCs (1 x 10(7) to 2 x 10(7) cells) were infused intravenously, and SPECT imaging was performed initially and on days 1, 2, 7 and 14. High quality SPECT images were obtained through 2 weeks of imaging. High initial MSC localization occurred in the lungs and slow progressive accumulation occurred in the liver, spleen and bone marrow. Renal activity was mild and persistent throughout imaging. No appreciable accumulation occurred in the myocardium. It is concluded that 111In oxine radiolabelling of MSCs is feasible, and in vivo imaging with SPECT provides a non-invasive method for sequentially monitoring cell trafficking with good spatial resolution. Because intravenous administration of MSCs results in significant lung activity that obscures the assessment of myocardial cell trafficking, alternative routes of administration should be investigated for this application.
间充质干细胞（MSCs）如果能成功输送到心肌梗死的目标部位，已显示出治疗潜力。本初步研究的目的是在猪心肌梗死模型中测试静脉注射后用111铟-奥克辛标记MSCs及单光子发射计算机断层扫描（SPECT）成像的可行性。成年农场猪（n = 2）接受了闭式胸腔实验性心肌梗死。静脉注射111铟-奥克辛标记的MSCs（1×10⁷至2×10⁷个细胞），并在初始时以及第1、2、7和14天进行SPECT成像。通过为期2周的成像获得了高质量的SPECT图像。MSCs最初在肺部大量定位，在肝脏、脾脏和骨髓中缓慢进行性蓄积。在整个成像过程中肾脏活性轻微且持续存在。心肌中未出现明显蓄积。结论是，用111铟-奥克辛对MSCs进行放射性标记是可行的，并且SPECT体内成像提供了一种具有良好空间分辨率的用于连续监测细胞转运的非侵入性方法。由于静脉注射MSCs会导致显著的肺部活性，从而模糊了对心肌细胞转运的评估，因此对于该应用应研究其他给药途径。
