Materials Science and Technology of Polymers, Faculty of Science and Technology and MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
Small. 2010 Dec 20;6(24):2870-6. doi: 10.1002/smll.201000713.
Detection of an analyte via supramolecular host-guest binding and quantum dot (QD)-based fluorescence resonance energy transfer (FRET) signal transduction mechanism is demonstrated. Surface patterns consisting of CdSe/ZnS QDs functionalized at their periphery with β-cyclodextrin (β-CD) were obtained by immobilization of the QDs from solution onto glass substrates patterned with adamantyl-terminated poly(propylene imine) dendrimeric "glue." Subsequent formation of host-guest complexes between vacant β-CD on the QD surface and an adamantyl-functionalized lissamine rhodamine resulting in FRET was confirmed by fluorescence microscopy, spectroscopy, and fluorescence lifetime imaging microscopy (FLIM).
通过超分子主客体结合和基于量子点（QD）的荧光共振能量转移（FRET）信号转导机制来检测分析物。通过将 QD 从溶液中固定在带有金刚烷末端的聚（丙稀亚胺）树枝状“胶”图案的玻璃基底上，获得了在其外围功能化有β-环糊精（β-CD）的 CdSe/ZnS QD 的表面图案。随后，通过荧光显微镜、光谱和荧光寿命成像显微镜（FLIM）证实了 QD 表面上的空β-CD 与金刚烷功能化的丽春红之间形成主客体配合物，从而导致 FRET。
