Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, PR China.
Talanta. 2012 Sep 15;99:409-14. doi: 10.1016/j.talanta.2012.05.072. Epub 2012 Jun 7.
In this paper, a novel optical biosensor for amantadine (AD) determination has been constructed successfully based on the fluorescence resonance energy transfer (FRET) between water-soluble β-Cyclodextrin (β-CD)-functionalized CdTe quantum dots (QDs) and Rhodamine B (RB). RB could enter the cavity of β-CD by hydrophobic interaction, and the process of FRET between QDs and RB occurred. However, the process of FRET was switched off with the addition of AD, due to its larger hydrophobic association constant with β-CD than that of RB. The fluorescence intensity of CdTe QDs (donor) would increase gradually with the increasing concentration of AD, which shown a good linear relationship in the range of 1×10(-5)-1.6×10(-4) mol/L with a correlation coefficient R(2)=0.998. We also obtained a satisfactory result using this spectrophotometric method for the determination of AD in pharmaceutical formulation. Furthermore, β-CD-functionalized CdTe QDs with AD in the cavity were incubated with target HepG2 cells and could be observed in the cytoplasm of cells. The β-CD-functionalized CdTe QDs could act as a visible biomarker for AD in cancer cells fluorescence imaging, which presents a potential application in biomedical field.
本文成功构建了一种基于水溶性β-环糊精（β-CD）功能化的CdTe 量子点（QDs）与罗丹明 B（RB）之间荧光共振能量转移（FRET）的新型金刚烷胺（AD）测定的光学生物传感器。RB 可以通过疏水相互作用进入β-CD 的腔中，并且发生 QDs 和 RB 之间的 FRET 过程。然而，随着 AD 的加入，FRET 过程被关闭，因为它与β-CD 的疏水缔合常数大于 RB。CdTe QDs（供体）的荧光强度会随着 AD 浓度的增加而逐渐增加，在 1×10(-5)-1.6×10(-4) mol/L 的范围内呈现出良好的线性关系，相关系数 R(2)=0.998。我们还使用这种分光光度法对药物制剂中的 AD 进行了测定，得到了令人满意的结果。此外，腔内含有 AD 的β-CD 功能化 CdTe QDs 与靶标 HepG2 细胞孵育后，可在细胞质中观察到。β-CD 功能化的 CdTe QDs 可作为癌细胞荧光成像中 AD 的可见生物标志物，这在生物医学领域具有潜在的应用。
