Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, Denmark.
Proteomics. 2010 Nov;10(22):3997-4011. doi: 10.1002/pmic.201000312. Epub 2010 Nov 2.
Quo Vadis: where are you going? Advances in MS-based proteomics have enabled research to move from obtaining the basic protein inventory of cells and organelles to the ability of monitoring their dynamics, including changes in abundance, location and various PTMs. In this respect, the cellular plasma membrane is of particular interest, by not only serving as a barrier between the "cell interior" and the external environment, but moreover by organizing and clustering essential components to enable dynamic responses to internal and external stimuli. Defining and characterizing the dynamic plasma membrane proteome is crucial for understanding fundamental biological processes, disease mechanisms and for finding drug targets. Protein identification, characterization of dynamic PTMs and protein-ligand interactions, and determination of transient changes in protein expression and composition are among the challenges in functional proteomic studies of the plasma membrane. We review the recent progress in MS-based plasma membrane proteomics by presenting key examples from eukaryotic systems, including mammals, yeast and plants. We highlight the importance of enrichment and quantification technologies required for detailed functional and comparative analysis of the dynamic plasma membrane proteome.
你要去哪里？基于 MS 的蛋白质组学的进展使研究能够从获得细胞和细胞器的基本蛋白质清单发展到监测它们的动态，包括丰度、位置和各种翻译后修饰的变化。在这方面，细胞质膜特别有趣，它不仅作为“细胞内部”和外部环境之间的屏障，而且通过组织和聚集基本成分来实现对内部和外部刺激的动态响应。定义和描述动态质膜蛋白质组对于理解基本的生物学过程、疾病机制以及寻找药物靶点至关重要。蛋白质鉴定、动态翻译后修饰和蛋白质-配体相互作用的特征描述，以及蛋白质表达和组成的瞬时变化的确定，都是质膜功能蛋白质组学研究中的挑战。我们通过展示真核系统（包括哺乳动物、酵母和植物）中的关键实例，回顾了基于 MS 的质膜蛋白质组学的最新进展。我们强调了详细的功能和比较分析动态质膜蛋白质组所需的富集和定量技术的重要性。
