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Rapid Commun Mass Spectrom. 2010 Dec 30;24(24):3527-30. doi: 10.1002/rcm.4804.
Atmospheric pressure ionization (API) sources have the ability to serve as dynamic 'reaction vessels' and this capability has been rapidly evolving over the past few years. With API sources, many different reagents and source conditions can be rapidly explored with minimal carryover or contamination of the mass spectrometer. While most API applications involve the simple protonation/deprotonation of analyte molecules, a great deal of flexibility and utility is available in almost any API source, provided the reagents and ionization conditions are judiciously selected. Here, the generation of a unique and useful class of reagent ions at atmospheric pressure is demonstrated for the first time. Within the AP gas-phase environment of a dual inlet ion source, the gas-phase synthesis of substituted N-phenylpyridinium cations is demonstrated by establishing conditions favorable to the nucleophilic substitution reaction required for their formation. The flexibility of this API source as a reaction vessel is also demonstrated.
大气压电离 (API) 源具有充当动态“反应容器”的能力，在过去几年中，这种能力发展迅速。通过 API 源，可以快速探索许多不同的试剂和源条件，而对质谱仪的交叉污染或携带污染最小。虽然大多数 API 应用涉及分析物分子的简单质子化/去质子化，但在几乎任何 API 源中都具有很大的灵活性和实用性，只要试剂和电离条件得到明智选择。在这里，首次展示了在大气压下生成独特且有用的一类试剂离子的方法。在双入口离子源的 AP 气相环境中，通过建立有利于形成它们所需的亲核取代反应的条件，证明了取代的 N-苯基吡啶鎓阳离子的气相合成。该 API 源作为反应容器的灵活性也得到了证明。
