基于着丝粒周围物质（PCM）在减数分裂纺锤体中的距离评估小鼠中期 II 卵母细胞的成熟能力：卵母细胞成熟过程中的 PCM 距离。
Evaluation of maturation competence of metaphase II oocytes in mice based on the distance between pericentriolar materials of meiotic spindle: distance of PCM during oocyte maturation.
机构信息
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai, 981-8555, Japan.
出版信息
J Assist Reprod Genet. 2011 Feb;28(2):157-66. doi: 10.1007/s10815-010-9496-2. Epub 2010 Nov 17.
PURPOSE
To ascertain whether metaphase II (MII) spindle shape influences oocyte competence, we examined the meiotic spindle organization in in vivo ovulated (IVO) oocytes and in spontaneously matured or follicle stimulating hormone (FSH)-induced oocytes.
METHODS
FSH-induced oocytes matured in Waymouth's MB752/1 or human tubal fluid (HTF) media and oocytes matured spontaneously in the basal medium were obtained, and spindles were detected by immunofluorescence. To evaluate the fertilization-associated differences in spindle morphology, we performed in vitro fertilization and analysed integrin mRNA expression.
RESULTS
The distance between the pericentriolar materials (PCMs) in oocytes matured under all conditions was initially more, but it reduced gradually and increased again thereafter. Therefore, oocytes exhibiting a reduction in the distance between PCMs had the highest development rate to blastocyst in each condition.
CONCLUSION
These results indicate that the 'maturation competence' of MII oocytes can be evaluated on the basis of the distance between PCMs.
目的
为了确定中期 II（MII）纺锤体形状是否影响卵母细胞的能力，我们检查了体内排出的（IVO）卵母细胞和自发成熟或卵泡刺激素（FSH）诱导的卵母细胞的减数分裂纺锤体组织。
方法
在 Waymouth's MB752/1 或人输卵管液（HTF）培养基中诱导 FSH 成熟的卵母细胞和在基础培养基中自发成熟的卵母细胞，并通过免疫荧光检测纺锤体。为了评估与纺锤体形态相关的受精差异，我们进行了体外受精并分析了整合素 mRNA 的表达。
结果
在所有条件下成熟的卵母细胞的中心体周围物质（PCM）之间的距离最初较大，但逐渐减少，然后再次增加。因此，在每种条件下，PCM 之间距离减小的卵母细胞具有最高的囊胚发育率。
结论
这些结果表明，可以根据 PCM 之间的距离评估 MII 卵母细胞的“成熟能力”。
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