Wang Qiang, Sun Qing-Yuan
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China.
Reprod Fertil Dev. 2007;19(1):1-12. doi: 10.1071/rd06103.
Mounting evidence that oocyte quality profoundly affects fertilisation an d subsequent embryo development drives the continued search for reliable predictors of oocyte developmental competence. In the present review, we provide an overall summary and analysis of potential criteria that can be used to evaluate oocyte quality. These criteria are specifically classified as morphological and cellular/molecular predictors. Traditional methods for the evaluation of oocyte quality are based on morphological classification of thefollicle, cumulus-oocytecomplex, polar body and/or meiotic spindle. Although the use of morphological characteristics as predictors of oocyte quality is controversial, such a grading system can provide valuable information for the preselection of oocytes with higher developmental competence and, therefore, may maximise embryo developmental outcome. Several intrinsic markers (such as mitochondrial status and glucose-6-phosphate dehydrogenase 1 activity) and extrinsic markers (such as apoptosis of follicular cells and levels of the transforming growth factor-beta superfamily in follicular fluid or serum) have also been reported as useful indicators of oocyte competence and embryo quality. Compared with the morphological parameters, these cellular and molecular predictors of oocyte quality may prove to be more precise and objective, although further studies and refinement of techniques are needed.
越来越多的证据表明，卵母细胞质量会深刻影响受精及随后的胚胎发育，这推动了人们继续寻找可靠的卵母细胞发育能力预测指标。在本综述中，我们对可用于评估卵母细胞质量的潜在标准进行了全面总结和分析。这些标准具体分为形态学预测指标和细胞/分子预测指标。评估卵母细胞质量的传统方法基于卵泡、卵丘-卵母细胞复合体、极体和/或减数分裂纺锤体的形态学分类。尽管将形态学特征用作卵母细胞质量的预测指标存在争议，但这样的分级系统可为预选具有较高发育能力的卵母细胞提供有价值的信息，因此可能使胚胎发育结果最大化。一些内在标志物（如线粒体状态和葡萄糖-6-磷酸脱氢酶1活性）和外在标志物（如卵泡细胞凋亡以及卵泡液或血清中转化生长因子-β超家族的水平）也已被报道为卵母细胞能力和胚胎质量的有用指标。与形态学参数相比，这些卵母细胞质量的细胞和分子预测指标可能更精确、更客观，不过仍需要进一步研究和技术改进。
