Yu Gang, Wu Xiying, Kilroy Gail, Halvorsen Yuan-Di C, Gimble Jeffrey M, Floyd Z Elizabeth
Stem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA, USA.
Methods Mol Biol. 2011;702:29-36. doi: 10.1007/978-1-61737-960-4_3.
Murine models of obesity or reduced adiposity are a valuable resource for understanding the role of adipocyte dysfunction in metabolic disorders. Primary adipocytes grown in culture and derived from murine adipose tissue are essential for studying the mechanisms underlying adipocyte development and function. Herein, we describe methods for the isolation, expansion, and long-term storage of murine adipose-derived stem cells along with a protocol for inducing adipogenesis in this cell population.
肥胖或脂肪减少的小鼠模型是理解脂肪细胞功能障碍在代谢紊乱中作用的宝贵资源。在培养中生长且源自小鼠脂肪组织的原代脂肪细胞对于研究脂肪细胞发育和功能的潜在机制至关重要。在此，我们描述了小鼠脂肪来源干细胞的分离、扩增和长期储存方法，以及在该细胞群体中诱导脂肪生成的方案。
