Yu Gang, Floyd Z Elizabeth, Wu Xiying, Halvorsen Yuan-Di C, Gimble Jeffrey M
Stem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA, USA.
Methods Mol Biol. 2011;702:17-27. doi: 10.1007/978-1-61737-960-4_2.
Adipose tissue is as an abundant and accessible source of stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Here, we describe methods from our own laboratory and the literature for the isolation and expansion of adipose-derived stem cells (ASCs). We present a large-scale procedure suitable for processing >100-ml volumes of lipoaspirate tissue specimens by collagenase digestion and a related procedure suitable for processing adipose tissue aspirates without digestion.
脂肪组织是一种丰富且易于获取的干细胞来源，具有多能特性，适用于组织工程和再生医学应用。在此，我们描述了来自我们自己实验室及文献中关于脂肪来源干细胞（ASC）分离和扩增的方法。我们介绍了一种适用于通过胶原酶消化处理超过100毫升抽脂组织标本的大规模程序，以及一种适用于处理未经消化的脂肪组织抽吸物的相关程序。
