Yu Gang, Floyd Z Elizabeth, Wu Xiying, Hebert Teddi, Halvorsen Yuan-Di C, Buehrer Benjamin M, Gimble Jeffrey M
Stem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA, USA.
Methods Mol Biol. 2011;702:193-200. doi: 10.1007/978-1-61737-960-4_14.
The primary physiological function of adipose-derived stem cells (ASCs) is to differentiate into adipose tissue. It is now possible to isolate, expand, and cryopreserve ASC from adipose depots of many animal species. These ASC can be induced to undergo adipogenic differentiation in vitro by exposure to a cocktail of chemical agents or inductive growth factors. The current chapter describes methods to induce adipogenesis and to quantify this differentiation process in vitro.
脂肪来源干细胞（ASC）的主要生理功能是分化为脂肪组织。现在可以从许多动物物种的脂肪库中分离、扩增和冻存ASC。通过暴露于化学试剂或诱导生长因子的混合物中，这些ASC可在体外被诱导进行脂肪生成分化。本章描述了在体外诱导脂肪生成并量化该分化过程的方法。
