Tucker H Alan, Bunnell Bruce A
Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, LA, USA.
Methods Mol Biol. 2011;702:121-31. doi: 10.1007/978-1-61737-960-4_10.
One of the hallmark characteristics of human adipose-derived stem cells (hASCs) is their ability to differentiate into cells of mesenchymal lineages. It is also becoming apparent that ASCs can mediate a therapeutic benefit through cytokine, paracrine-driven mechanisms influencing apoptosis, angiogenesis, and potent anti-inflammatory responses. Although there is still no clear consensus on the antigen expression pattern that will define hASCs, a protocol is also presented for the flow cytometric analysis utilizing a series of antibody panels. The analysis of these surface epitope patterns can aide in the isolation and characterization of hASCs. Moreover, using this standardized antibody panel, direct comparisons can be made between ASCs isolated from various tissue sources, which will benefit the field by providing uniformity to the comparison process.
人脂肪来源干细胞（hASCs）的一个标志性特征是它们能够分化为间充质谱系的细胞。越来越明显的是，脂肪来源干细胞可以通过细胞因子、旁分泌驱动机制介导治疗益处，影响细胞凋亡、血管生成和强大的抗炎反应。尽管对于定义hASCs的抗原表达模式仍未达成明确共识，但本文还介绍了一种使用一系列抗体组合进行流式细胞术分析的方案。对这些表面表位模式的分析有助于hASCs的分离和表征。此外，使用这种标准化的抗体组合，可以对从各种组织来源分离的脂肪来源干细胞进行直接比较，这将为比较过程提供一致性，从而使该领域受益。
