Pal Pallabi, Medina Abelardo, Chowdhury Sheetal, Cates Courtney A, Bollavarapu Ratna, Person Jon M, McIntyre Benjamin, Speed Joshua S, Janorkar Amol V
Department of Biomedical Materials Science, School of Dentistry, University of Mississippi Medical Center, 2500 N State Street, Jackson, MS 39216, USA.
Division of Plastic Surgery, Department of Surgery, University of Mississippi Medical Center, 2500 N State Street, Jackson, MS 39216, USA.
Biomedicines. 2024 May 1;12(5):997. doi: 10.3390/biomedicines12050997.
Clinical and basic science applications using adipose-derived stem cells (ADSCs) are gaining popularity. The current adipose tissue harvesting procedures introduce nonphysiological conditions, which may affect the overall performance of the isolated ADSCs. In this study, we elucidate the differences between ADSCs isolated from adipose tissues harvested within the first 5 min of the initial surgical incision (well-vascularized, nonpremedicated condition) versus those isolated from adipose tissues subjected to medications and deprived of blood supply during elective free flap procedures (ischemic condition). ADSCs isolated from well-vascularized and ischemic tissues positively immunostained for several standard stem cell markers. Interestingly, the percent change in the CD36 expression for ADSCs isolated from ischemic versus well-vascularized tissue was significantly lower in males than females ( < 0.05). Upon differentiation and maturation to adipocytes, spheroids formed using ADSCs isolated from ischemic adipose tissue had lower triglyceride content compared to those formed using ADSCs isolated from the well-vascularized tissue ( < 0.05). These results indicate that ADSCs isolated from ischemic tissue either fail to uptake fatty acids or fail to efficiently convert those fatty acids into triglycerides. Therefore, more robust ADSCs suitable to establish in vitro adipose tissue models can be obtained by harvesting well-vascularized and nonpremedicated adipose tissues.
使用脂肪来源干细胞（ADSCs）的临床和基础科学应用正日益受到欢迎。当前的脂肪组织采集程序会引入非生理条件，这可能会影响分离出的ADSCs的整体性能。在本研究中，我们阐明了在手术初始切口的前5分钟内采集的脂肪组织（血管丰富、未用药状态）中分离出的ADSCs与在择期游离皮瓣手术期间经药物处理且缺乏血液供应的脂肪组织（缺血状态）中分离出的ADSCs之间的差异。从血管丰富和缺血组织中分离出的ADSCs对几种标准干细胞标志物呈阳性免疫染色。有趣的是，与血管丰富组织相比，从缺血组织中分离出的ADSCs的CD36表达变化百分比在男性中显著低于女性（<0.05）。在分化并成熟为脂肪细胞后，与使用从血管丰富组织中分离出的ADSCs形成的球体相比，使用从缺血脂肪组织中分离出的ADSCs形成的球体甘油三酯含量更低（<0.05）。这些结果表明，从缺血组织中分离出的ADSCs要么无法摄取脂肪酸，要么无法有效地将这些脂肪酸转化为甘油三酯。因此，通过采集血管丰富且未用药的脂肪组织，可以获得更适合建立体外脂肪组织模型的健壮ADSCs。
