Decaunes Pauline, Estève David, Zakaroff-Girard Alexia, Sengenès Coralie, Galitzky Jean, Bouloumié Anne
Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Médecine Moléculaire de Rangueil, Université Toulouse III Paul-Sabatier, Toulouse, France.
Methods Mol Biol. 2011;702:151-61. doi: 10.1007/978-1-61737-960-4_12.
The present method describes an immunoselection/depletion approach to isolate the native human adipose tissue-derived progenitor cells that are free from endothelial cells and immune cells by the use of magnetic nanobeads and microbeads coupled to antibodies. Moreover, methods to isolate and to analyse the distinct cell populations that constitute the microenvironment of the human adipose tissue progenitor cells, i.e. mature adipocytes, endothelial cells, and macrophages, are mentioned.
本方法描述了一种免疫选择/去除方法，通过使用与抗体偶联的磁性纳米珠和微珠来分离不含内皮细胞和免疫细胞的天然人脂肪组织来源的祖细胞。此外，还提到了分离和分析构成人脂肪组织祖细胞微环境的不同细胞群体的方法，即成熟脂肪细胞、内皮细胞和巨噬细胞。
