Froelich C J, Wallman J, Skosey J L, Teodorescu M
Department of Medicine, University of Illinois, Chicago 60612.
J Rheumatol. 1990 Feb;17(2):192-200.
An ELISA panel assay (ANA/6) which simultaneously detects IgG autoantibodies against 6 antigens (ssDNA, dsDNA, Sm, RNP/Sm, SSA, and SSB) was evaluated with sera from 98 patients with systemic lupus erythematosus (SLE) and related conditions, 68 disease controls, who were positive or negative by fluorescent ANA (FANA), and 100 healthy controls. The antigen panel specifically identified the particular autoantibodies present and had a high level of sensitivity which was reflected by the frequency of detection of autoantibodies in different patient groups. All active patients with SLE were positive for at least one autoantibody and 80% of these patients had 3 or more autoantibodies compared to only 20% of inactive patients. A large heterogeneity in antibody profiles was also noted. About 90% of patients with positive FANA, but no detectable autoimmune rheumatic disorder, i.e., false positive, were negative by the panel. A new method of standardization was designed to use the ANA/6 quantitatively. Very large differences in the level of all 6 autoantibodies were observed between sera from patients with active and inactive SLE, suggesting a potential usefulness of ANA/6 for patient management.
采用酶联免疫吸附试验组合检测法（ANA/6）对98例系统性红斑狼疮（SLE）患者及相关疾病患者、68例荧光抗核抗体（FANA）检测呈阳性或阴性的疾病对照患者以及100例健康对照者的血清进行检测，该方法可同时检测针对6种抗原（单链DNA、双链DNA、Sm、核糖核蛋白/Sm、SSA和SSB）的IgG自身抗体。抗原组合能够特异性识别存在的特定自身抗体，且具有较高的敏感性，这一点通过不同患者组中自身抗体的检测频率得以体现。所有活动期SLE患者至少有一种自身抗体呈阳性，其中80%的患者有3种或更多自身抗体，而不活动期患者这一比例仅为20%。抗体谱也存在很大的异质性。约90%的FANA检测呈阳性但未检测到自身免疫性风湿性疾病（即假阳性）的患者，该检测组合结果为阴性。设计了一种新的标准化方法以对ANA/6进行定量检测。活动期和不活动期SLE患者血清中所有6种自身抗体水平存在很大差异，提示ANA/6在患者管理方面可能具有一定作用。
