Maddison P J, Skinner R P, Vlachoyiannopoulos P, Brennand D M, Hough D
Clin Exp Immunol. 1985 Nov;62(2):337-45.
The profile of autoantibodies to four soluble cellular ribonucleoproteins nRNP, Sm Ro(SSA) and La(SSB) was determined using ELISA with immuno-affinity-purified antigens in a connective tissue disease population. Compared with immunodiffusion, results using ELISA showed greater sensitivity but lower specificity and low titres of antibodies were frequently found in the sera of patients with connective tissue diseases other than systemic lupus erythematosus. This was true even for antibodies to Sm which have been considered highly specific for SLE. Antibodies to these antigens were predominantly of the IgG class and were capable of fixing complement irrespective of the clinical context. As previously demonstrated by immunodiffusion strong associations between anti-nRNP and anti-Sm and between anti-Ro(SSA) and anti-La(SSB) were detected by ELISA, while antibodies to nRNP and to Ro(SSA) identify distinctive serological groups. The observation that certain antibodies are closely linked suggests a relationship between the immune responses to particular antigens, and this might be explained by biological links between the antigens.
在结缔组织病患者群体中，使用免疫亲和纯化抗原的酶联免疫吸附测定（ELISA）法测定了针对四种可溶性细胞核糖核蛋白nRNP、Sm、Ro（SSA）和La（SSB）的自身抗体谱。与免疫扩散法相比，ELISA法的检测结果显示出更高的敏感性，但特异性较低，并且在除系统性红斑狼疮之外的结缔组织病患者血清中经常发现低滴度抗体。即便对于被认为对系统性红斑狼疮具有高度特异性的抗Sm抗体而言亦是如此。针对这些抗原的抗体主要为IgG类，并且无论临床情况如何均能够固定补体。正如先前通过免疫扩散法所证实的，ELISA法检测到抗nRNP与抗Sm之间以及抗Ro（SSA）与抗La（SSB）之间存在强关联，而抗nRNP和抗Ro（SSA）抗体可识别不同的血清学组。某些抗体紧密相关这一观察结果提示了针对特定抗原的免疫反应之间存在关联，这或许可以通过抗原之间的生物学联系来解释。
