Department of Chemistry, Nankai University, Tianjin, PR China.
J Sep Sci. 2010 Dec;33(23-24):3786-93. doi: 10.1002/jssc.201000337.
A novel molecularly imprinted polymer monolith was prepared by the room temperature ionic liquid-mediated in situ molecular imprinting technique, using norfloxacin (NOR) as the template, methacrylic acid as the functional monomer, ethylene glycol dimethacrylate as the cross-linker. The optimal synthesis conditions and recognition properties of NOR-imprinted monolithic column were investigated. The results indicated that the imprinted monoliths exhibited good ability of selective recognition against the template and its structural analog. Using the fabricated material as solid-phase extraction sorbent, a sample pre-treatment procedure of molecularly imprinted solid-phase extraction coupling with HPLC was developed for determination of trace quinolone residues in animal tissues samples. The recoveries ranging from 78.16 to 93.50% for eight quinolones antibiotics such as marbofloxacin, NOR, ciprofloxacin, danofloxacin, difloxacin, oxolinic acid, flumequine and enrofloxacin were obtained.
一种新型的分子印迹聚合物整体柱是通过室温离子液体介导的原位分子印迹技术制备的，以诺氟沙星（NOR）为模板，甲基丙烯酸为功能单体，乙二醇二甲基丙烯酸酯为交联剂。考察了 NOR 印迹整体柱的最佳合成条件和识别性能。结果表明，印迹整体柱对模板及其结构类似物具有良好的选择性识别能力。采用制备的材料作为固相萃取吸附剂，建立了高效液相色谱法测定动物组织样品中痕量喹诺酮类抗生素残留的分子印迹固相萃取前处理方法。8 种喹诺酮类抗生素（马波沙星、诺氟沙星、环丙沙星、丹诺沙星、二氟沙星、氧氟沙星、盐酸洛美沙星和恩诺沙星）的回收率在 78.16%至 93.50%之间。
