He Chunji, Wu Moutian
National Antidoping Laboratory, China Antidoping Agency, 1st An Ding Lu, Beijing, 100029, China.
Bioanalysis. 2009 Aug;1(5):953-65. doi: 10.4155/bio.09.85.
Detection of doping with recombinant human growth hormone is one of the challenges for antidoping analysis. This review focuses on the most important relevant publications that provide insight into the laboratory measurement of human growth hormone (hGH), antibodies and standards, the isoform approach and the biomarker approach. The isoform approach monitors the changes of hGH molecular isoform composition in serum and was applied at the Olympic Games in Athens in 2004, Turin in 2006 and Beijing in 2008. The markers approach detects a formula score, which reflects the changes in concentration of IGF-1 and P-III-P. All these methodologies measure the concentrations of growth hormone and its isoforms for isoform approach, or the concentrations of IGF-1 and P-III-P. All factors that affect these measurements should be taken into account for the development of methods to detect doping with recombinant hGH.
检测重组人生长激素兴奋剂是反兴奋剂分析面临的挑战之一。本综述重点关注最重要的相关出版物，这些出版物深入探讨了人生长激素（hGH）、抗体和标准品的实验室测量、异构体方法和生物标志物方法。异构体方法监测血清中hGH分子异构体组成的变化，并在2004年雅典奥运会、2006年都灵冬奥会和2008年北京奥运会上应用。标志物方法检测一个公式得分，该得分反映了IGF-1和P-III-P浓度的变化。所有这些方法都测量生长激素及其异构体的浓度（异构体方法），或IGF-1和P-III-P的浓度。在开发检测重组hGH兴奋剂的方法时，应考虑所有影响这些测量的因素。
