Fernandes A J, Diotaiuti L, Dias J C, Romanha A J, Chiari E
Centro de Pesquisas René Rachou, Belo Horizonte, Brasil.
Mem Inst Oswaldo Cruz. 1989 Jan-Mar;84(1):87-93. doi: 10.1590/s0074-02761989000100016.
Out of 87 opossums, Didelphis albiventris, captured in the Bambuí area (Minas Gerais State), 32 (36.7%) were found infected by Trypanosoma cruzi; the rates varied according to whether the specimens originated from sylvan, rural peridomiciliar or urban surroundings, being 34.9, 81.8 and 7.7 respectively. From 20 of the infected opossums the anal glands were repeatedly examined and found positive in only one (5%) specimen (GA 9), with 7 positive examinations out of 17 performed through an 18-months periods. Material from these glands produced patent parasitemia in opossums and sub-patent infections in mice. Isolates from the opossum GA 9, obtained through xenodiagnoses and hemocultures and from cultures of the infected anal glands fitted into zymodeme Z1.
在从班布伊地区(米纳斯吉拉斯州)捕获的87只白腹负鼠(Didelphis albiventris)中,发现32只(36.7%)感染了克氏锥虫;感染率因样本来源不同而有所差异,这些样本分别来自森林、农村周边地区或城市环境,感染率分别为34.9%、81.8%和7.7%。对20只感染负鼠的肛腺进行了多次检查,仅在1只(5%)样本(GA 9)中呈阳性,在18个月的时间里进行的17次检查中有7次呈阳性。这些腺体的材料在负鼠中产生了明显的寄生虫血症,在小鼠中产生了亚临床感染。通过异种接种诊断、血液培养以及感染肛腺培养从负鼠GA 9中分离出的菌株属于酶谱型Z1。