Xu Raymond Naxing, Rieser Matthew J, El-Shourbagy Tawakol A
Dept. R46W, Bldg. AP13A-2, Abbott Laboratories, Abbott Park, IL 60064-66126, USA.
Bioanalysis. 2009 Apr;1(1):239-53. doi: 10.4155/bio.09.12.
Hydrophilic interaction chromatography (HILIC) has, in recent years, been shown to be an important supplement to reversed-phase liquid chromatography for polar analytes. HILIC, in conjunction with tandem mass spectrometry (MS/MS), has been steadily gaining acceptance in the analysis of polar compounds from complex biological matrices. This hyphenated technique offers the advantages of improved sensitivity by employing high organic content in the mobile phase, shortened sample preparation time with direct injection of the organic-solvent extracts of biological samples and the potential for ultra-fast analysis because of low-column backpressure. This article reviews recent challenges presented by HILIC, advancements in the better understanding of retention characteristics of analytes with different mobile- and stationary-phase compositions and solutions to ion suppression and interference problems encountered in HILIC-MS/MS assays. Applications of HILIC-MS/MS are summarized, including those for pharmacokinetic studies, metabolic studies, therapeutic drug monitoring and clinical diagnostics.
近年来，亲水作用色谱法（HILIC）已被证明是反相液相色谱法分析极性分析物的重要补充。HILIC与串联质谱法（MS/MS）相结合，在分析复杂生物基质中的极性化合物方面越来越受到认可。这种联用技术具有诸多优势，例如通过在流动相中采用高有机相含量提高灵敏度、直接进样生物样品的有机溶剂提取物从而缩短样品制备时间，以及由于柱背压低而具备超快速分析的潜力。本文综述了HILIC近期面临的挑战、在更好地理解不同流动相和固定相组成下分析物保留特性方面的进展，以及解决HILIC-MS/MS分析中遇到的离子抑制和干扰问题的方法。总结了HILIC-MS/MS的应用，包括药代动力学研究、代谢研究、治疗药物监测和临床诊断等方面的应用。
