Deng Pan, Chen Xiaoyan, Zhong Dafang
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China.
Bioanalysis. 2009 Apr;1(1):187-203. doi: 10.4155/bio.09.19.
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has played an important role in quantitative bioanalytical assays. This review summarizes the recent progress on quantification of polar drugs in plasma with LC-MS/MS. Various types of polar analytes were extracted using protein precipitation or solid-phase extraction and precolumn derivatization was utilized in some cases. The analytes were then separated using different types of chromatographic method, which included reversed-phase chromatography, aqueous normal-phase chromatography, hydrophilic interaction liquid chromatography and ion-pairing chromatography. Stationary phases of mixed mode and porous graphitic carbon materials are gaining acceptance in bioanalytical applications. These technologies can be valuable supplements in the quantification of polar drugs in human plasma with LC-MS/MS. Matrix effects have also been discussed in this review.
液相色谱-串联质谱法（LC-MS/MS）在定量生物分析测定中发挥了重要作用。本综述总结了近年来利用LC-MS/MS对血浆中极性药物进行定量分析的研究进展。采用蛋白沉淀或固相萃取法提取各类极性分析物，某些情况下还会使用柱前衍生化。然后，使用不同类型的色谱方法分离分析物，包括反相色谱法、水相正相色谱法、亲水作用液相色谱法和离子对色谱法。混合模式固定相和多孔石墨化碳材料在生物分析应用中越来越受到认可。这些技术对于利用LC-MS/MS定量分析人血浆中的极性药物而言是很有价值的补充。本综述还讨论了基质效应。
