Department of Chemical and Biological Engineering, Women and Children's Hospital of Buffalo, University at Buffalo, State University of New York, Amherst, New York 14260-4200, USA.
Tissue Eng Part A. 2011 Apr;17(7-8):981-90. doi: 10.1089/ten.tea.2010.0109. Epub 2011 Jan 16.
Our laboratory recently reported a new source of smooth muscle cells (SMCs) derived from hair follicle (HF) mesenchymal stem cells. HF-SMCs demonstrated high proliferation and clonogenic potential as well as contractile function. In this study, we aimed at engineering the vascular media using HF-SMCs and a natural biomaterial, namely small intestinal submucosa (SIS). Engineering functional vascular constructs required application of mechanical force, resulting in actin reorganization and cellular alignment. In turn, cell alignment was necessary for development of receptor- and nonreceptor-mediated contractility as soon as 24 h after cell seeding. Within 2 weeks in culture, the cells migrated into SIS and secreted collagen and elastin, the two major extracellular matrix components of the vessel wall. At 2 weeks, vascular reactivity increased significantly up to three- to fivefold and mechanical properties were similar to those of native ovine arteries. Taken together, our data demonstrate that the combination of HF-SMCs with SIS resulted in mechanically strong, biologically functional vascular media with potential for arterial implantation.
我们实验室最近报道了一种新的平滑肌细胞（SMCs）来源，即毛囊（HF）间充质干细胞。HF-SMCs 表现出高增殖和克隆形成潜力以及收缩功能。在这项研究中，我们旨在使用 HF-SMC 和天然生物材料，即小肠黏膜下层（SIS）来构建血管中层。构建功能性血管结构需要施加机械力，从而导致肌动蛋白重组和细胞排列。反过来，细胞排列对于受体和非受体介导的收缩性的发展是必要的，这在细胞接种后 24 小时内就发生了。在培养的 2 周内，细胞迁移到 SIS 中，并分泌胶原蛋白和弹性蛋白，这是血管壁的两个主要细胞外基质成分。在 2 周时，血管反应性显著增加了 3 到 5 倍，机械性能与天然绵羊动脉相似。总之，我们的数据表明，HF-SMC 与 SIS 的结合产生了机械强度高、生物学功能强的血管中层，具有动脉植入的潜力。
