Hoogduijn Martin J, Gorjup Erwin, Genever Paul G
Department of Biology, University of York, YO10 5YW, York, UK.
Stem Cells Dev. 2006 Feb;15(1):49-60. doi: 10.1089/scd.2006.15.49.
We compared the growth and differentiation characteristics of hair follicle-derived dermal stem cells with bone marrow mesenchymal stem cells (MSCs). Follicular dermal cells were isolated from whisker hairs of Wistar rats and bone marrow MSCs were isolated from femora of the same animals. The adherent hair follicle dermal cells showed a fibroblastic morphology in serum-containing culture medium, were CD44(+), CD73(+), CD90(+), and CD34(), and had a population doubling time of 27 h. MSCs isolated from the bone marrow showed a similar morphology and population doubling time and expressed the same cell-surface markers. Following exposure to appropriate induction stimuli, both cell populations had the capacity to differentiate into various mesenchymal lineages, such as osteoblasts, adipocytes, chondrocytes, and myocytes and expressed neuroprogenitor cell markers. The rate and extent of differentiation were remarkably similar for both hair follicleand bone marrow-derived cells, whereas interfollicular dermal cells failed to differentiate. We identified telomerase activity in follicle dermal stem cells and marrow MSCs and demonstrated that they were capable of clonal expansion. In ex vivo analyses, we identified the presence of putative dermal stem cells in the dermal sheath and dermal papillae of the hair follicle. Consequently, the hair follicle may represent a suitable, accessible source for MSCs.
我们比较了毛囊来源的真皮干细胞与骨髓间充质干细胞（MSCs）的生长和分化特性。从Wistar大鼠的须毛中分离出毛囊真皮细胞，并从同一动物的股骨中分离出骨髓间充质干细胞。贴壁的毛囊真皮细胞在含血清的培养基中呈现成纤维细胞形态，CD44(+)、CD73(+)、CD90(+)，CD34(-)，群体倍增时间为27小时。从骨髓中分离出的间充质干细胞表现出相似的形态和群体倍增时间，并表达相同的细胞表面标志物。在暴露于适当的诱导刺激后，这两种细胞群体都有能力分化为各种间充质谱系，如成骨细胞、脂肪细胞、软骨细胞和肌细胞，并表达神经祖细胞标志物。毛囊来源的细胞和骨髓来源的细胞在分化速率和程度上非常相似，而毛囊间真皮细胞则无法分化。我们在毛囊真皮干细胞和骨髓间充质干细胞中鉴定出端粒酶活性，并证明它们能够进行克隆扩增。在体外分析中，我们在毛囊的真皮鞘和真皮乳头中鉴定出假定的真皮干细胞的存在。因此，毛囊可能是间充质干细胞的一个合适的、可获取的来源。
