Groeger Sabine, Doman Eugen, Chakraborty Trinad, Meyle Jörg
Dental School, Department of Periodontology, Justus-Liebig-University of Giessen, Germany.
Eur J Oral Sci. 2010 Dec;118(6):582-9. doi: 10.1111/j.1600-0722.2010.00782.x. Epub 2010 Oct 14.
The gingival epithelium plays an important role in the protection of oral tissues from microbial challenge. Oral keratinocytes form various cellular contacts, including tight junctions, and thus are able to create an epithelial barrier. A measurable indicator of barrier function in vitro is the transepithelial electrical resistance (TER). Porphyromonas gingivalis is recognized as a major aetiologic agent of periodontal disease and exhibits a variety of virulence factors. The aim of the study was to investigate the effect, in vitro, of infection with P. gingivalis on gingival barriers composed of primary and immortalized human keratinocytes. Primary and immortalized human gingival keratinocytes were infected with different strains of P. gingivalis. The impact of the bacterial challenge on the barrier was analysed by measuring the TER. The destructive effects of gingipains were blocked by specific enzyme inhibitors. After an initial increase of about 20-30% in infected wells, the TER decreased to zero. Gingipain inhibitors delayed the destruction of the barrier by 12 ± 4 h. In all cases, the loss of TER was accelerated if the system was infected from the basolateral side. A distinct effect of P. gingivalis on the epithelial barrier function of three-dimensional cultured epithelial cell models was demonstrated, which can partly be attributed to the activity of gingipains.
牙龈上皮在保护口腔组织免受微生物侵害方面发挥着重要作用。口腔角质形成细胞形成各种细胞连接，包括紧密连接，因此能够形成上皮屏障。体外屏障功能的一个可测量指标是跨上皮电阻（TER）。牙龈卟啉单胞菌被认为是牙周病的主要病原体，并表现出多种毒力因子。本研究的目的是在体外研究牙龈卟啉单胞菌感染对由原代和永生化人角质形成细胞组成的牙龈屏障的影响。用不同菌株的牙龈卟啉单胞菌感染原代和永生化人牙龈角质形成细胞。通过测量TER分析细菌攻击对屏障的影响。牙龈蛋白酶的破坏作用被特异性酶抑制剂阻断。在感染孔中最初增加约20 - 30%后，TER降至零。牙龈蛋白酶抑制剂将屏障的破坏延迟了12±4小时。在所有情况下，如果从基底外侧感染系统，TER的丧失会加速。证明了牙龈卟啉单胞菌对三维培养上皮细胞模型的上皮屏障功能有明显影响，这部分可归因于牙龈蛋白酶的活性。
