Department of Prosthodontics and Periodontology, Piracicaba Dental School, State University of Campinas, Campinas, São Paulo, Brazil.
Gerodontology. 2012 Jun;29(2):e186-91. doi: 10.1111/j.1741-2358.2010.00439.x. Epub 2010 Nov 17.
This study evaluated the influence of the area of Candida albicans biofilm on denture disinfection by microwave energy.
Candida albicans biofilm was allowed to form for 72 h on resin discs, and three small coverage or seven large coverage discs were placed onto the palatal surface of sterile maxillary dentures. Each denture was immersed in 200 ml distilled water and individually irradiated at a power of 450, 630 or 900 W for different time intervals (1, 2 or 3 min) (n = 6). The effectiveness of disinfection was evaluated by counting the residual cells. The data were analysed by anova and Tukey's HSD test (α = 0.05). Pearson's correlation test was performed to determine the correlation between effectiveness of sterilisation and temperature.
Dentures with a larger area of biofilm demanded a longer irradiation exposure to achieve disinfection (p < 0.001), irrespective of power setting, and in this time no yeast growth was detected. Dentures with small areas of biofilm were disinfected after 1 min at 900 W and 2 min at 450 or 630 W. A positive correlation was found between water temperature and effectiveness of disinfection (r = 0.6170; p < 0.001).
The C. albicans biofilm area influenced disinfection by microwave energy; therefore dentures with larger biofilm areas required longer irradiation exposure to be disinfected.
本研究评估了白色念珠菌生物膜面积对微波能量消毒义齿的影响。
让白色念珠菌生物膜在树脂盘上形成 72 小时，然后将三个小覆盖面积或七个大覆盖面积的圆盘放置在无菌上颌义齿的腭表面上。每个义齿都浸泡在 200ml 蒸馏水中，并分别以 450、630 或 900W 的功率照射不同的时间间隔（1、2 或 3 分钟）（n = 6）。通过计数残留细胞来评估消毒效果。使用方差分析和 Tukey 的 HSD 检验（α = 0.05）分析数据。进行 Pearson 相关性检验以确定消毒效果与温度之间的相关性。
不论功率设置如何，具有较大生物膜面积的义齿都需要更长的照射暴露时间才能实现消毒（p < 0.001），在此时间内未检测到酵母生长。具有小面积生物膜的义齿在 900W 照射 1 分钟和在 450 或 630W 照射 2 分钟后即可消毒。水温与消毒效果呈正相关（r = 0.6170；p < 0.001）。
白色念珠菌生物膜面积影响微波能量消毒；因此，具有较大生物膜面积的义齿需要更长的照射暴露时间才能消毒。
