Laboratori de Microbiologia Sanitària i Mediambiental (MSM Lab)-Aquasost, UNESCO Chair in Sustainability, Universitat Politècnica de Catalunya, 08222 Terrassa, Barcelona, Spain.
Helicobacter. 2010 Oct;15(5):473-6. doi: 10.1111/j.1523-5378.2010.00794.x.
While Helicobacter pylori exists in a bacillary form in both the natural habitat and the human host, detrimental environmental circumstances have been observed to lead to the conversion of H. pylori from the bacillary to the coccoid form. However, the viability or nonviability of coccoid forms remains to be established in H. pylori. The aim of this study was to determine whether the quantitative PCR combined with propidium monoazide could be an alternative and good technique to determine H. pylori viability in environmental samples and, to contribute to understanding of the role of the H. pylori forms.
Viability, morphological distribution, and the number of live H. pylori cells were determined using a propidium monoazide-based quantitative PCR method, at various time points.
Under adverse environmental conditions was observed the conversion of H. pylori from the bacillary to the coccoid form, and the decrease in amplification signal, in samples that were treated with propidium monoazide, over the time.
Incorporation of propidium monoazide indicates that there is an increase in H. pylori cells with the damaged membrane over the study, leading to the manifestation of cellular degeneration and death. Consequently, quantitative PCR combined with propidium monoazide contributes to our understanding of the role of H. pylori cells, under adverse environmental conditions.
尽管幽门螺杆菌在自然栖息地和人类宿主中都以杆菌形式存在，但已观察到不利的环境情况会导致幽门螺杆菌从杆菌形式转化为球菌形式。然而，幽门螺杆菌球菌形式的生存能力或非生存能力仍有待确定。本研究旨在确定定量聚合酶链反应结合吖啶橙是否可以作为一种替代技术，用于确定环境样本中幽门螺杆菌的生存能力，并有助于了解幽门螺杆菌形态的作用。
使用基于吖啶橙的定量聚合酶链反应方法，在不同时间点确定生存能力、形态分布和活幽门螺杆菌细胞的数量。
在不利的环境条件下，观察到幽门螺杆菌从杆菌形式向球菌形式的转化，并且在用吖啶橙处理的样本中，随着时间的推移，扩增信号减少。
吖啶橙的结合表明，在研究过程中，细胞膜受损的幽门螺杆菌细胞数量增加，导致细胞退化和死亡的表现。因此，定量聚合酶链反应结合吖啶橙有助于我们了解幽门螺杆菌细胞在不利环境条件下的作用。
